Dear All,
I'm trying to clone a minigene (three exons and the two introns in between, total is 4.2 kb) in a pcDNA 3,1(+). Unfortunately without success: no insert inside the vector after transformation. I checked all the routine controls for cloning but I'm wondering if I'm using the proper vector. Maybe pcDNa 3.1 is to small for such insert. Any idea and comments are more than welcome.
Thank you so much in advance!
MC
Christian Gabriel
Hi MC,
I see no reasons why the vector should not work for this. Cloning can always be tricky. If you want recommendations, you need to provide more information: How do you amplify your insert, does this work fine, which restriction enzymes do you use etc...
best
c.
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