Hello everyone,
I am struggling to find a better way to test the transparency of my hydrogels made of kappa carrageenan and/or with salts. I tried testing a 2cm thick hydrogel but the absorptance value reached above 1. I haven't tried using a cell with a liquid phase of the hydrogel.
I'd be glad to read your suggestions.
Thanks!
Balram Lohan
Prepare the hydrogel samples: Create hydrogel samples of the desired size and shape. Ensure that the samples are uniform in thickness and free from any visible defects. Set up the spectrophotometer: Turn on the UV-VIS spectrophotometer and allow it to warm up. Follow the manufacturer's instructions to set the wavelength range appropriate for your analysis (typically UV or visible range). Blank measurement: Perform a baseline measurement by placing a blank reference sample (usually a cuvette containing the solvent or medium used in the hydrogel preparation) into the spectrophotometer. This measurement helps correct for any background interference or absorbance by the solvent or medium. Sample measurement: Replace the blank reference sample with the hydrogel sample. Ensure that the sample is properly positioned in the cuvette holder, aligning it with the light path of the spectrophotometer. Scan the spectrum: Scan the spectrum by measuring the absorbance or transmission of light at different wavelengths across the desired range (e.g., 200-800 nm). The spectrophotometer will provide data on the intensity of transmitted or absorbed light. Analyze the results: Examine the collected data to assess the transparency of the hydrogel. Transparency is indicated by high transmission values and low absorbance values. Typically, a transparent hydrogel will exhibit high transmission across a broad wavelength range. Compare with control/reference: To evaluate the transparency of the hydrogel, compare the obtained transmission spectrum with that of a control/reference sample. The control/reference sample can be a hydrogel with known transparency or the solvent/medium used in the hydrogel preparation. Repeat and average: For accurate results, repeat the measurement multiple times with different samples from the same batch. Take multiple measurements at different locations within each sample and calculate the average transmission values to ensure consistency.
To effectively test the transparency of hydrogels using a UV-VIS spectrophotometer, you can follow these general steps:
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John Mc Guiller Daarol Calma
hello Balram Lohan it seems that this is an answer from chatgpt. It would be great if you can share something that is based on published papers for references. thanks!
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