Which is the best mitochondrial genome annotation tool? Can anyone provide the list?

I have sequenced the mitochondrial genome of some moths species. I want to annotate and the sequence will submit in the NCBI.

19 November 2020 8,849 1 View

Lithium ion cell holder for Samsung 21700 - 50E cells?

Hello everyone, I have 5 Samsung 21700 - 50 E (NCM chemistry) cells. I want to do basic charge and discharge by connecting it in series. Does anyone know if there is a cell holder available...

05 October 2020 6,133 3 View

Which is the first complete mitochondrial genome sequenced lepidopteran Moth species?

I am preparing a research article about the complete mitogenome of moth. I want to include the details in my research article.

20 September 2020 8,151 3 View

Installation cost of a smart grid or Vehicle2grid ?

Hello, Does anyone know the cost to install Vehicle2grid service at home or commercial places ? I found a rough price as 5000$ in internet, but could anyone recommend a much cheaper solutions or...

07 July 2019 5,677 3 View

Is Oxidation of the PDMS surface necessary before silanizing it with APTES?

How imortant is it to oxidise the surface for silanization? I see some methods that use silnization buffer containing ethanol to activate the APTES and used it directly on PDMS without oxidation.

18 October 2018 5,806 3 View

Function in Scilab similar to Matlab xlswrite ?

Hi, Similar to Matlab xlswrite(filename,A), what is the function name in Scilab to write on a new excel file ? I tried searching, but couldn't find out. There was only write_csv...

25 April 2018 5,190 1 View

Scilab plot window not generated ?

Hi, When I try to plot in Scilab, no graphic window is generated (see pic). This is a simple plot between two columns of one matrix. Sometimes, I get error saying 'invalid index'. Does anyone know...

21 February 2018 3,548 3 View

Why capacitance value of 2RC battery model increases with temperature ?

18 September 2017 2,170 1 View

Why battery model fit goes bad with increase in C-rate ?

I am using 2RC Equivalent circuit model to fit discharge current pulses of LFP cell. I use 4 pulses (8C, 4C, 2C, 1C). The RMSE is good for 2C and 1C~5mV, but for 4C and 8C it seems to increase...

11 September 2017 6,960 3 View

How to fit current pulse data to impedance data

I have a set of discharge current pulse values (Voltage vs Time) as well as Impedance Spectroscopy data of Li-ion battery. How could I transfer these pulses to frequency domain and fit with other...

29 June 2017 5,590 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View