Which is the best mitochondrial genome annotation tool? Can anyone provide the list?

I have sequenced the mitochondrial genome of some moths species. I want to annotate and the sequence will submit in the NCBI.

19 November 2020 8,849 1 View

Lithium ion cell holder for Samsung 21700 - 50E cells?

Hello everyone, I have 5 Samsung 21700 - 50 E (NCM chemistry) cells. I want to do basic charge and discharge by connecting it in series. Does anyone know if there is a cell holder available...

05 October 2020 6,133 3 View

Which is the first complete mitochondrial genome sequenced lepidopteran Moth species?

I am preparing a research article about the complete mitogenome of moth. I want to include the details in my research article.

20 September 2020 8,151 3 View

Installation cost of a smart grid or Vehicle2grid ?

Hello, Does anyone know the cost to install Vehicle2grid service at home or commercial places ? I found a rough price as 5000$ in internet, but could anyone recommend a much cheaper solutions or...

07 July 2019 5,677 3 View

Is Oxidation of the PDMS surface necessary before silanizing it with APTES?

How imortant is it to oxidise the surface for silanization? I see some methods that use silnization buffer containing ethanol to activate the APTES and used it directly on PDMS without oxidation.

18 October 2018 5,806 3 View

Function in Scilab similar to Matlab xlswrite ?

Hi, Similar to Matlab xlswrite(filename,A), what is the function name in Scilab to write on a new excel file ? I tried searching, but couldn't find out. There was only write_csv...

25 April 2018 5,190 1 View

Scilab plot window not generated ?

Hi, When I try to plot in Scilab, no graphic window is generated (see pic). This is a simple plot between two columns of one matrix. Sometimes, I get error saying 'invalid index'. Does anyone know...

21 February 2018 3,548 3 View

Why capacitance value of 2RC battery model increases with temperature ?

18 September 2017 2,170 1 View

Why battery model fit goes bad with increase in C-rate ?

I am using 2RC Equivalent circuit model to fit discharge current pulses of LFP cell. I use 4 pulses (8C, 4C, 2C, 1C). The RMSE is good for 2C and 1C~5mV, but for 4C and 8C it seems to increase...

11 September 2017 6,960 3 View

How to fit current pulse data to impedance data

I have a set of discharge current pulse values (Voltage vs Time) as well as Impedance Spectroscopy data of Li-ion battery. How could I transfer these pulses to frequency domain and fit with other...

29 June 2017 5,590 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View

Repository for capture-seq raw reads?

I am a research tech in a wildlife genetics group and my PI and I are trying to figure out how/where to put raw reads in a repository. We have capture-seq NGS from Rapid Genomics. The post-doc who...

17 February 2021 7,575 2 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View