I am writing a research article using complete mitochondrial genome sequences. I need to reconstruct the phylogenetic tree using the MrBayes.
https://www.youtube.com/watch?v=Wr7SDCI8X-k
Hello everyone. I am trying to analyze the NOx study for my cementitious TiO2 material. According to the ISO standard, I have to follow 1000 ppb and 3 L/min of working fluid. In my case, I have a...
17 January 2024 7,266 0 View
explain TOF.
23 March 2023 4,968 1 View
I want to reconstruct the phylogenetic tree using the maximum parsimony method through MEGA X software. Which substitution model do I have to choose. kindly give me the details.
29 November 2021 4,191 3 View
I have 3 scales in a survey form. Each scales ranges from 1 to 10 which measure the severity, occurrence and identification ratings. This is for conducting Failure Mode effect Analysis by...
27 September 2021 5,583 2 View
I have 9 questions based out of 5-point Likert Scale coming under one construct in order to measure customer's perception towards supply chain. I have 151 data points given by both male and...
25 September 2021 7,146 3 View
The questionnaire was trying to explore the customer priorities leading to their purchase of a product (More like, if your are intending to purchase this, what shall be your priorities in a ranked...
14 September 2021 6,087 4 View
I have a ranked data where the respondents have ranked a set of 7 variables ( 1 to 7 ). what are the techniques to ensure the reliability, validity of such ranked dataset in general if any?. what...
14 September 2021 3,123 4 View
Hello everyone, All lithium ion battery pack/system in the Electric vehicle (EV) needs to achieve certain certification before entering the country's market. So does anyone know all the standards,...
20 June 2021 10,140 4 View
Dear Researchers While I am reconstructing the phylogenetic tree, I am getting an error message like this "Aligned sequences must be equal lengths (in line 3133). There was no error in the...
17 May 2021 2,250 5 View
Hello everyone, All lithium ion battery pack/system in the Electric vehicle (EV) needs to achieve certain certification before entering the country's market. So does anyone know all the...
09 May 2021 7,800 2 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View
Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...
28 July 2024 6,938 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View