Hi everyone, we find a new protein showing no any similarity with any other proteins. The encoding gene has many copies and so we can't study it by knocking out it. Is this case (have no similarity with other proteins) common in eukaryotes? And how should we study it?
Hello Qinglin
I am assuming you might find this thread helpful
https://www.researchgate.net/post/How_to_study_about_a_novel_protein_and_its_function_if_there_is_no_database_or_antibody_is_available_to_study_the_expression_profile_of_that_protein
Qinglin Yang, maybe you have to perform an NGS analysis from your sample to figure out what it's the target from this protein. There's a lot of possibilities in that case, but maybe a CHIP-seq analysis will be a good option.
Have you ever worked with these analyses? ChIP-Seq leverages next-generation sequencing (NGS) to quickly and efficiently determine the distribution and abundance of DNA-bound protein targets of interest across the genome.
https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-chip.html
Good luck!
Garima Ayachit, thanks for your imformation. It's useful. Maybe I can try to discuss the possibility with a structural biologist.
A functional inhibition (e.g. via siRNAs or inhibitory antibodies) could help to identify its biological functions.
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