How to inhibit a powerful nuclease?

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Hanna Alalam

multiple rounds of phenol choloform extraction. Or you can use proteinase K prior to extraction.

Qinglin Yang

Hi Hanna, I lost all DNA in the first phenol choloform extraction... that's why I called the nucleases "powerful"...@Hanna Alalam

Ok then add the proteinase k directly to the lysis solution and see if that helps.