How many Cas9 molecules are sufficient for gene editing in one cell?

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Noor Edan Jarad

hi see this link

https://www.ncbi.nlm.nih.gov/pmc/articles

Eirini Kalliara

I would recommend reading this paper, "Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery" by Lin et al. eLife 2014. It really helped me.

Qinglin Yang

Hi, Jarad, I am sorry I can't find the paper at this link. Could you give me the title of this paper?

Thank you Kalliara. I've read this paper and it's helpful to me. But for the question, maybe I have to find some data about the injection of Cas9-RNP into single cell.

Thank you Riese, it's very helpful.