How many Cas9 molecules are sufficient for gene editing in one cell?

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Eirini Kalliara

I would recommend reading this paper, "Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery" by Lin et al. eLife 2014. It really helped me.

Qinglin Yang

Thank you Kalliara. I've read this paper and it's helpful to me. But for the question, maybe I have to find some data about the injection of Cas9-RNP into single cell.