How to identify the replication origin of a plasmid in Naegleria?

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Yezhi Fu

Hi Qinglin,

I attached a file that may help you.

Charles Graham Clark

Unfortunately, eukaryotic origins of replication are not easily identified based on sequence data and the ones that have been identified differ between organisms. It is possible that you may find a region with unusual characteristics, -like an inverted repeat, a different G+C composition, a 'strand switch' in base composition or the ability to form a hairpin loop - but without functional analysis it would be impossible to conclude that this was the origin of replication.