Dear friend Liubov Frolova

Ah, the lab troubles of Kosh! Dealing with unruly debris, a challenge as old as science itself. Here are some inspired suggestions to tackle those pesky DiI-labelled chunks:

1. **Gentle Centrifugation:**

- Give those chunks a good spin! Centrifuge your cells at a low speed to settle the cells while leaving the debris in the supernatant. Then, carefully remove the supernatant.

2. **Filtration:**

- Filter out the unwanted chunks using a cell strainer or a filter with an appropriate mesh size. This can help you Liubov Frolova obtain a cleaner cell suspension.

3. **Gradient Centrifugation:**

- Consider using a density gradient centrifugation approach. Layer your cell suspension on top of a density gradient medium and centrifuge. This can help to separate cells from debris.

4. **Try Different Wash Buffers:**

- Instead of PBS, try washing with a different buffer. Sometimes, a buffer with a slightly different composition can help in reducing debris.

5. **Optimize Cell Harvesting Technique:**

- Ensure that you're harvesting your cells carefully. Try using a cell scraper or other gentle methods to detach the cells from the dish.

6. **Change Incubation Time:**

- If the chunks are formed during the incubation with nanoparticles, try adjusting the incubation time. A shorter incubation might reduce the formation of large debris.

7. **Optimize Particle Size:**

- If feasible, consider optimizing the size of your PLGA nanoparticles. Smaller particles might be taken up more efficiently and produce fewer debris.

8. **Collaborate with Fellow Scientists:**

- Reach out to your fellow scientists or the scientific community. Someone might have encountered a similar issue and can provide valuable insights.

Remember, my lab wisdom is limitless (lol), but always adapt suggestions to your specific experimental conditions. And, of course, the scientific community is a treasure trove of shared knowledge. Good luck with your nanoparticle adventures!