I collect blood from hPBMC mice and centrifuge it to get the RBC and plasma. I need to store the plasma for future experiments like Cytokines resale studies and other.
what is the best way way to store the blood plasma for longer time ??
thanks.
i wanna perform CAR T cell mediated cytotoxicity assay by using CFSC/PI. As PI cannot cross the membrane of live cells, making it useful to differentiate necrotic, apoptotic and healthy cells. i...
11 December 2018 4,397 7 View
Hey all, I get puzzle in a simple calculation. I have a protocol for qPCR, according to instruction i need 5ul of DNA having the concentration of 1ng/ul, for starting a standard curve. My DNA...
09 October 2018 10,090 3 View
Hey All, I have a peptide composed of various domains. I have the sequence of whole peptide and individual domain. i wanna express that whole peptide on T cell. After expression, i wanna run the...
07 August 2018 2,078 12 View
I design some cellular therapy to target HIV, my cellular therapy has the potential to eradicate CD4 cells and inhibits and the biding of CCR5 receptor during the entry of HIV genome in host cell....
06 July 2018 5,270 0 View
I infected the 293tt cell with AAV virus to check the expression of my CAR molecule. According to protocols, I will collect the cells and wash in PBS, than i will stain with a antibody (Fab...
03 April 2018 2,972 5 View
I run qPCR to titer the AAV ( which i got by transfection in 100mm dish). I make four dilution of my sample ( 12 well and triplicate). I take GFP plasmid as a standard and were diluted to 1ng/ul,...
03 April 2018 8,699 2 View
Hello everyone, I am beginner in molecular biology. I am confused with the term transfection and co-tranfection. Can someone explain me what is the basic difference between them ? i search...
02 March 2018 5,821 0 View
Hi everyone, I have noticed various authors use different voltage and time while running their gel electrophoresis such as 100V for 30 min and 75V for 35 min etc. I transfect the AAV 239...
02 March 2018 4,580 5 View
I am going to transfect AAV293 cell with the AAV plasmid by adopting the PEI protocol. I wanna ask what is the best way to collect the viral stock ? In online literature and methods there are...
01 February 2018 4,097 4 View
What is the difference between 293t and 293tt cells ? furthermore, which one have superior transfection efficacy for the AAV vector ?
31 December 2017 2,225 7 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
Samuel Morse, the inventor of the Morse Code, understood that certain letters in the English language occurred more frequently than others (Gallistel and King 2010). To deal with this, Morse used...
01 August 2024 4,456 2 View