Waqas Nawaz

11 Questions 21 Answers 0 Followers

Questions related from Waqas Nawaz

How to induced apoptosis/Necrosis to get high% for PI positive group in CFSE/PI mediated CAR-T cytotoxicty assay ?

i wanna perform CAR T cell mediated cytotoxicity assay by using CFSC/PI. As PI cannot cross the membrane of live cells, making it useful to differentiate necrotic, apoptotic and healthy cells. i...

12 December 2018 4,353 7 View

How to Calculate the concentration of DNA plasmid from stock for qPCR ?

Hey all, I get puzzle in a simple calculation. I have a protocol for qPCR, according to instruction i need 5ul of DNA having the concentration of 1ng/ul, for starting a standard curve. My DNA...

10 October 2018 10,012 3 View

How to calculate molecular weight of protien from a sequence ?

Hey All, I have a peptide composed of various domains. I have the sequence of whole peptide and individual domain. i wanna express that whole peptide on T cell. After expression, i wanna run the...

08 August 2018 2,019 12 View

Cell line to target CD4 and CCR5 ?

I design some cellular therapy to target HIV, my cellular therapy has the potential to eradicate CD4 cells and inhibits and the biding of CCR5 receptor during the entry of HIV genome in host cell....

07 July 2018 5,220 0 View

How to store Blood plasma ?

I collect blood from hPBMC mice and centrifuge it to get the RBC and plasma. I need to store the plasma for future experiments like Cytokines resale studies and other. what is the best way way...

05 May 2018 1,291 0 View

How to calculate the Genome copy number of AAV titer by qPCR ?

I run qPCR to titer the AAV ( which i got by transfection in 100mm dish). I make four dilution of my sample ( 12 well and triplicate). I take GFP plasmid as a standard and were diluted to 1ng/ul,...

04 April 2018 8,638 2 View

How to stain and fix cell for flow cytometry for long time ??

I infected the 293tt cell with AAV virus to check the expression of my CAR molecule. According to protocols, I will collect the cells and wash in PBS, than i will stain with a antibody (Fab...

04 April 2018 2,914 5 View

Transfection and Co-transfection ?

Hello everyone, I am beginner in molecular biology. I am confused with the term transfection and co-tranfection. Can someone explain me what is the basic difference between them ? i search...

03 March 2018 5,766 0 View

How to determine the optimum voltage and time for a better separation in gel electrophoresis and westron blotting ?

Hi everyone, I have noticed various authors use different voltage and time while running their gel electrophoresis such as 100V for 30 min and 75V for 35 min etc. I transfect the AAV 239...

03 March 2018 4,540 5 View

How to prepare AAV viral stock after transfection?

I am going to transfect AAV293 cell with the AAV plasmid by adopting the PEI protocol. I wanna ask what is the best way to collect the viral stock ? In online literature and methods there are...

02 February 2018 4,038 4 View

What is the difference between 293t and 293tt cells ? and which one have superior transfection efficacy ?

What is the difference between 293t and 293tt cells ? furthermore, which one have superior transfection efficacy for the AAV vector ?

01 January 2018 2,177 7 View