Hi Everyone,
Does anyone have experience in working with haemocytes of P. vannamei or P. monodon? Could you please suggest me any effective method to prevent cell clumping when taking haemolymph out from shrimp?
Thank you so much.
Thao
This is not for your species, but should help. Addition of some EDTA calcium/magnesium chelator, adjusted to neutral (pH7) or haemolyph pH should work. Add 1-2 drops of a 2% solution per ml. If the clumping occurs as you harvest the haemolymph, try starting with a small amount of 0.5% EDTA or EGTA in saline in the syringe or pipette You use to collect your sample.
Ellen A G Chernoff thank you so much for your answer. I will try and see if it works for shrimp. Much appreciated.
The composition of the anticoagulant is given in the following paper
https://www.sciencedirect.com/science/article/abs/pii/S014111361100002X
You may also check for local availability of Sodium Citrate, K2EDTA or Lithium Heparin microtainer tube (0.5-1.0 mL). They are quite handy as you may not require to dilute the haemocytes. The fish world is very diverse, each animal with various biological characteristics.
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