hello,

I am working on rccs. I started with 12 rpm speed for 5 ml and 10 ml vessel in RCCs for glial cells ( doubling time:24 hrs) and seeding density of 2*105, but while harvesting I can see clustures of cells with naked eye. So, I want to ask how to standardize the speed and seeding density for a particular type of cell in RCCS. Many thanks