How to stabilize insects for tissue dissection and RNA extraction?

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Acid digestion techniques: Microwave digestion of samples containg ~10%glycerol with concentrated HNO3 (?!)?

Hello, I need your advice as I am rather new to acid digestion techniques. I am trying to build up an ICP method for the analysis of lithium. However, there appears to be interferences,...

23 June 2021 4,380 0 View

What is the proper puromycin concentration for the skpc1 cell line?

I have transfected the skpc1 cell line, unfortunately in the lab here noone has done a puromycin concentration curve yet to assess 100 % killing of untransfected cells. Since i have not enough...

18 March 2021 4,952 2 View

Which practical guide presenting the non-parametric tests ?

Dear colleagues, I am looking for a practical guide presenting the non-parametric tests intended for students without mathematical background (or very little) with if possible the codes SAS or R....

24 September 2020 5,841 3 View

Does anyone know of a 3D insect tracking system for a large environment (5x2x2m)?

Hi, I'm looking for a 3D tracking system to reconstruct the trajectories of flying bumblebees in a white environment with a few plants. The size of the environment is ~5x2x2m. I am struggling to...

26 August 2020 3,599 5 View

I have developed a python package to combine results from multiple classifiers (eg: signal peptide, beta-barrel fold). Can you give me a feedback?

Hello everyone! A few years ago i have developed an algorithm to combine the results from multiple classifiers (ex: signal peptide predictors, beta-barrel predictors) in a consensus by using an...

23 August 2020 5,774 3 View

How to generate merge of Western Blot (chemiluminescent) and corresponding marker (colorimetric)?

Dear researchers, I am recording my Western Blots on a Biorad Chemidoc System which results in one .scn file for the marker and on .scn file for the actual Western Blot signal. The coresponding...

05 May 2020 9,062 0 View

Who can provide Covid-19 / SARS treatment guidelines?

I currently have the WHO, the Chinese, and the German guideline for SARS induced by corona virus. I mean management in the ICU. Can anybody contribute a respective Italian or Spanish guideline?

17 March 2020 1,786 7 View

Why this have to measurment the electrochemical impedance spectroscopy(EIS) for measurement electrochemical performance?

I've read a jornal about rechargeable aluminum battery with CMK-3 cathode and I don't understand why they have to measurement EIS for performance measurement A super-long life rechargeable...

23 February 2019 5,833 4 View

Pre-dawn or midday water potential measurements to determine differences between water status of plants under contrasting water regimes?

Pre-dawn water potential is said to be a more reliable indicator of plant water status, since water potential is measured at a time when the plant is assumed to be in equilibrium with the soil...

06 February 2019 761 5 View

Does anyone know if it is possible to breed Apis cerana in Europe ?

I would like to know if it is possible to breed Apis cerana in Europe in order to merge it with Apis mellifera under controlled conditions of course.

06 February 2019 4,287 9 View

Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,686 1 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Melt curve problem in qPCR?

Do these peaks in the curve indicate primer dimer or non-specific products?

01 August 2024 7,466 0 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...

31 July 2024 5,645 2 View

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View