I want to develop Chiral HPLC method of compound having solubility only in DMSO. I can't use DMSO as a diluent to solubilize that compound.
If it's soluble in DMSO, you should try also dimethylformamide. The solubility will be lower, but still enough for HPLC analysis.
I already take solubility trails in DMF as well but not found good result.
Try to use a mixture of water/THF, both solvents are highly polar and can be used in HPLC
In my research field I got UV inactive compound having one Chiral Center I want to separate the enantiomers on HPLC having UV detector So what can i do to get UV response on HPLC.
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Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
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