In one of my experiments, for NAD-dependent deacetylase sirtuin 7, I use the commercial human recombinant sirtuin 7 from sigma. Now I want to know that compared with the untreated sirtuin7 in solution, whether the enzyme activity of experiment group with treated sirtuin7 in solution is downregulated or unchanged. Is there any simple and reasonable-price method for this kind of enzyme?
Thanks a lot~