I used PVPP (Polyvinyl-polypirrolidone) to remove tannins from a plant extract, but i would like to know if such complex can be dissolved to recover tannins.
Have to agree with Phillip (use 70% aq acetone, maybe with small % of acetic acid to limit oxidation), although you won't be dissolving the complex, rather desorbing the tannins from the PVPP (can be used as a stationary phase for separation of phenolics). Even still, some components might remain bound. I've used neat formic acid (followed by 95% aq methanol) to elute wine tannins bound to Oasis HLB (divinyl benzene/N-vinylpyrrolidone copolymer) which wouldn't budge with aq methanol or aq acetone. Could also try 0.1 M NaOH as eluent but I'd be wary of oxidation.
Thanks. What i´m trying to do is discard tannins and other polyphenolics as responsibles for the anthelmintic activity exhibited by a plant extract (dereplication). Then, i used PVPP to precipitate tannins and analize only the supernatant. But it might be interesting to see what kind of polyphenolics are present in the PVPP complex.
Always interesting to check out the polyphenols as they have a range of bioactivities. Maybe others have studied them for your particular plant but maybe not, so have a look at the literature.
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