What is the difference between master and doctoral degree?

Hello there.. I've recently submitted my master thesis in marketing. As a reseacher, I now know that the purpose of a master degree is to know academic research tools and how to do and write an...

09 February 2021 6,692 9 View

How can I reduce run-to-run variation in TMT MS experiments?

I planned an experimental setup using 10-plex TMT with 9 conditions and 1 bridge channel to compare 5 replicates. But since I experienced some run-to-run variation, I was wondering if a better...

31 January 2021 3,405 3 View

Can I use alpha amylase enzyme from (fungal source) if I need to characterize alpha amylase inhibitor from marine algae for hyperglycemia management?

I need to characterize alpha amylase enzyme from macroalgae for management of blood glucose level in diabetic patients, my question is can I use (fungal alpha amylase enzyme) in inhibition assay?

18 January 2021 735 4 View

How can I write a Scientific proposal to present it in public conference?

Writing Proposal

30 December 2020 6,783 3 View

Why RNA is totally degraded?

18 October 2020 5,252 22 View

How can i separate a polymer after a polymerization reaction from the unreacted monomers, taking in consideration that it's really sticky?

The polymer i'm fabricating will be used to make a delivery system, so it must be separated from the monomers right ? i tried to use solvent antisolvent method but the polymer is only soluble in...

13 October 2020 2,002 4 View

How to prepare 37% Formaldehyde from paraformaldehyde?

I want to prepare 37% formaldehyde for denatured formaldehyde agarose gel preparation (for RNA) How can I prepare 37% formaldehyde? And how long this solution would be stable?

11 October 2020 914 4 View

What is the concentration of saturated solution of bromophenol blue?

I want to know the concentration of a home-made saturated aqueous solution of Bromophenol blue. How could I know when I add unknown number of grams of the dye?

05 October 2020 3,423 3 View

What is the reason of introduction of SDM 6 nucleotides before the right position in a gene on a plasmid when sequenced?

I am trying to introduce SDM for a gene on a plasmid. After Pcr (with HF Q5 polymerase and primers designed by NEB website the length of one of them 19) , Dpn 1, ligation, transformation and...

27 September 2020 5,493 11 View

Can you please help me out?

Greetings! I am a Master's Student at IBA Karachi. This survey is purely for research purpose and its objective is to gather information about performance variables and constraints that emerge...

05 May 2020 7,068 2 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View