Hi everyone,
I did SDS-PAGE for a series of proteins that I was sure about them. In my SDS-PAGE was added TCE (2,2,2-trichloro ethanol) as stain free for proteins. After running the gel I took it and put on UV transluminator for giving uv wavelenght (about 250-360 nm) excitation, AFTER exposure time about 1-5 min, I deteced for emition but no sign with proteins bands and gel was clear.
What should I do for visiting my proteins bands under UV?
I had proteins bands when I was staining gel by commassie blue.
Do I need a specific chemoDOC instrument? or I can do it by typical UV transluminator?
Best wishes
Hi,
The signal of TCE is relatively low in that wavelength range (which of course depends on the structure of your protein), therefore it is better to detect the emission at 450 nm. If that does not work, maybe you can try conventionally staining your samples.
Thanks dear Arghavan,
I have forced to use this type of colour before doing westernblot.
Have you ever done this type of gel? Did you use to use stain free gel in a special instrument?
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