Fatemeh Vahdani

6 Questions 9 Answers 0 Followers

Questions related from Fatemeh Vahdani

Depletion igg from serum?

Hi everyone, I wanna get a suitable protocol for depletion of IgG and other immunoglobulins from serum, without kits or chromatography but with chemical methods like ammonium sulfate. Can anyone...

11 June 2024 9,400 2 View

How many sample of serum need to validate proteomics data ?

Hi everyone, I wanna do proteomics of monkeys serums in 2 states, healthy and patient. How many monkeys in each group I need to run proteomics and validate my data from it? Do I deplete albumin...

05 June 2024 4,026 3 View

How do I western blot of serum proteins without any unspecific binding of secondary antibody to my blot?

Hi everyone, I have some important problems with my western blot against serum proteins. I have used secondary antibody conjugated by HRP that binds in unspecific manner to other proteins as same...

04 June 2024 5,850 8 View

How to see proteins in SDS-PAGE with stain free (2,2,2-trichloro ethanol)?

Hi everyone, I did SDS-PAGE for a series of proteins that I was sure about them. In my SDS-PAGE was added TCE (2,2,2-trichloro ethanol) as stain free for proteins. After running the gel I took it...

05 November 2023 2,249 2 View

Is it possible to use SDS instead of LDS in LDS sample buffer ?

I want to do MALDI-TOF mass for my protein. For its procedure I need to do bis tris electrophoresis of immunoprecipitated protein, then excise protein band. For running bis tris electrophoresis, I...

08 June 2023 859 5 View

Which gel protein stain is the best alternative to instant blue for doing mass spect of a band of protein from bis tris gel?

Instant blue is a protein gel stain that suitable for mass spect procedure, but I want to replace it with another suitable stain for mass spect. what is your idea to change it?

08 June 2023 709 1 View