How to search lncRNA sequence for putative miRNA binding sites?

More Evan McCabe's questions See All

Is study location indexed in bibliometrics?

This seems essential, especially in ecology, but does not appear to be one of the metrics indexed by Web of Science or Scopus in published papers. Am I missing something? If not, can we start a...

17 October 2023 2,457 4 View

Can anyone give insight on the space charge layer in solid electrolyte-electrode interface?

Is it just a theoretical concept? If so, how is it modeled? Is it a consequence of poor physical contact?

01 August 2023 6,168 2 View

I seem to have accidentally created a duplicate account, can you delete it?

Hi - I accidentally just said "Yes" to something and seem to have created a duplicate account. Please delete the duplicate account.

18 April 2023 6,595 2 View

How can I decrease non-specific fluorescence in glutaraldehyde fixed tissue?

I am doing immunohistochemistry on auditory nerve fibers in the mouse brain. I am perfusing with 0.1% glutaraldehyde, 4% paraformaldehyde to fix the tissue, with the plan of taking confocal images...

13 March 2023 5,050 2 View

How can we prove the limit for the fibonacci sequence?

The Fibonacci numbers are defined with the recursive formula: F0 =1,F1 =1,Fn =Fn−1+Fn−2 I have tried using induction to show that Fn ≤ 1.7^(n) for all n ≥ 0. However, I got to a point where I was...

17 January 2023 8,066 8 View

Where can I find bilateral FDI data? UNCTAD FDI Database doesn't provide it anymore?

I am currently conducting a research regarding the effect of an FTA formation on the FDI of the FTA member states (which include ASEAN countries). I have read that people used to be able to find...

09 August 2022 4,266 4 View

Is my stain-free total protein normalization technique correct?

I am monitoring the expression of mitochondrial membrane-bound proteins in European pear peel tissue via quantitative fluorescent western blot analysis. I am normalizing on membrane with...

28 February 2022 8,072 1 View

How to implement ID3 algorithm for classification?

I am working on a data mining project. The goal of this project is to implement an ID3 classifier and Naive Bayes Classifer. I have to sets of data for ID3 the following test sets are provided A2...

15 November 2021 959 3 View

How to apply the Apriori Algorithm?

I am interested in understanding the applications behind the Apriori Algorithm. Given a datasource with a transaction id column and a list of items. How can we use the Apriori algorithm for...

04 November 2021 1,007 0 View

How to create a datawarehouse?

My goal in this project is create a datawarehouse. I am primarily using online communities and platforms to teach myself the concepts of data warehousing. For...

01 November 2021 3,234 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View