I'd like to identify crop varieties which are tolerant against salinity at field level. Say, I've 15 maize varieties which are sown on a RCB design with 3 replications. Among them I have to select variety(ies) which are tolerant to salinity (e.g. tolerant, moderate, susceptible). Noted soil salinity (ECe) at germination was 2.38 dS/m and at harvest >10 dS/m. After harvest, in terms of yield is it possible to select or categorize varieties against salinity through multivariate technique (e.g. PCA, biplot, cluster analysis)?
N.B.: I've read a paper (attached), where they used multivariate techniques as indirect method to select common bean against soil acidity.
Look at the following book under the topics, screening designs or Plackett-Burman. You can also just Google these two terms. Best wishes, David Booth
https://www.amazon.com/Response-Surface-Methodology-Optimization-Experiments/dp/1118916018/ref=sr_1_1?ie=UTF8&qid=1533804756&sr=8-1&keywords=response+surface+methodology
Yield is most important factor but exposing different cultivars to variable salt concentration of graded levels in.pot studies is good to generate preliminary data.
Yes.. You can do PCA/ bilpot or dendogram to classify different genotypes in groups according to the salinity responses. But, you may perform ANCOVA or multiple regression also for better understanding. Judging some physiological/ biochemical indices also give a clear understanding in this context. If you want, I can help you... Thanks...
PS. You may read the attached paper as an example of UPGMA analysis to cluster different root-stock of citrus.
Yield is the most imp parameter. But, from research point of view, associated factors are also important to identify. The use of PCA, PCA+cluster analysis, NLPCA approaches are widely used tools in this context.
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