Dear Abbas,

Please check the below given link and PDF attachment.

http://link.springer.com/article/10.1023/A:1008662313042

Good luck

 RS mostly form the sclerotia and you can perform primary screening by direct confrontation assay. 2nd you need to use slcerotia mortality test by using different spore suspension of your potent fungal bio agents. Generally Chitinase producing fungal bio-agents are potential against RS.

We find that Trichoderma harzianum is a good bio control agent against Rhizoctonia solani under arid soils of India. However it has been reported that Greek indigenous Streptomycetes is a good bio control agent against RS. Please consult:

www.ncbi.nlm.nih.gov/indigenous

How did you isolate that Biocontrol agents from soil? Is there any protocol? Protocol must be accurate.

hi

Hi,we find that streptomycetes consensus is Avery good biological control agent against rhizoctonia solani and other fungi

Hi Abbas, 

For specific isolation I would suggest baiting bag technique (using R. solani) where bait (sclerotia rapped in muslin cloth pouch) to be buried in the rhizosphere (desired sampling site), and it will attract the potential biocontrol organisms towards it and after few days you can collect  pouch along with surrounding soil and go for isolation. Attached is the relevant ref for same.

Article Selective isolation, evaluation and characterization of anta...

Dear All, If I have to isolate Actinomycetes, Please recommend a selective media for Actinomycetes, Suppose for streptomycetes consensus

You can use

1. starch casein agar

2. Actinomycetes isolation agar (readily available)

3. General medium with 3-5% glycerol added in it

4. By enriching soil sample with CaCO2 before isolation (1-2 days)

Good luck

Dear Abbas,

Here are the some recipes of culture media for Actinomycetes. Hope this will help you.

Kind regard