How to screen the drug for its anticancer activity in Drosophila?
Article An in vivo large-scale chemical screening platform using Dro...
I was trying to culture glial cell from embryonic day 17th. But, cells were not survived. I provided the DMEM+10% FBS and glutamax. Even though the cell are not differentiating into glial cells....
27 February 2023 1,497 1 View
I am trying to optimize the dose of drug for neuronal cell culture. How do I determine EC50 value? Suggest literature and easiest method and calculations. is checking the cell viability acceptable...
26 October 2022 6,108 1 View
Is there any method to convert median to mean of a data set?
06 September 2020 4,715 3 View
I came across one question that is it possible to make any changes in the published article by authors? If yes how...?
27 June 2020 6,230 10 View
In one way ANOVA I got a value of F = 12, the df numerator = 2 df denominator = 6. As per F distribution table, the critical F value is 3.46. P-value by the software is 0.0080. Then how to...
03 April 2020 8,803 0 View
During my PhD and Postdoc work, I have used manual patch clamp (Axon Instruments) with digidata 1440 ad multiclamp 700B amplifier. I’m somewhat confused right now so need your valuable...
17 July 2019 2,061 3 View
How to calculate dose of drugs to the animals if human dose is known.?
24 May 2019 3,743 3 View
I am working on primary neuronal culture. I have two drugs, suppose A and B. how can we determine various concentrations of those drugs have to be made to give a dose to the cells?
01 January 1970 6,390 4 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
EDX analisys
31 July 2024 5,947 2 View
I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...
29 July 2024 8,034 4 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...
26 July 2024 6,606 0 View
"Integration of Mass Media and Communication into the System of International Relations: Place, Role, Actuality and Prospects: Modern Media Issues 2024" Website: https://immcir.alnoor.edu.iq/
23 July 2024 3,139 6 View
Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?
22 July 2024 1,210 2 View