History/experience of technology / computing, who wants to participate?

i will write a book on the technological revolutions these last 40 years from a personal experience. who would like to join me and write a chapter?

18 January 2021 3,679 2 View

How would you scale intra-day returns to daily returns?

16 December 2020 6,284 3 View

Looking for help with identification of two MS-spectra of peaks detected in GCMS multiresidue pesticides analyses?

Dear colleagues, I am looking for some support in the identification of the MS-spectra of two unknown peaks detected in a GCMS multiresidue pesticides analyses of an extract of a water sample...

04 September 2020 3,137 0 View

Can I calculate a gene expression rate like this?

I want to calculate a gene expression rate of 1 gene of interest and 2 reference genes (GAPDH, 18S) over different timepoints and accounted for primer efficiency. Since im working with RNA...

06 December 2017 6,296 1 View

Whole Mount DAPI Staining

15 May 2017 4,754 2 View

No title

21 October 2015 7,299 3 View

Is there a tool to look for known or simply conserved promoter regions for non-coding RNA?

Predicting promoter regions for ncRNA in bacteria. I am looking for a promoter of a certain RNA I know the sequence and locus of.

01 September 2014 2,993 5 View

How can you find general similarities of enriched mRNAs obtained from single protein immunoprecipitation?

I discovered a few hundred mRNAs that are co-immunoprecipitated with my protein of interest using RIP-Seq. Now I want to find similarities based on sequence, structure, motifs etc. which could...

04 April 2013 5,382 2 View

miRNome and NGS?

I am planning to study the miRNomes in different diseases. Does anyone have experience with the analysis of miRNomes using NGS, especially when starting with a low amount (2-10 ng) of enriched...

26 August 2012 8,854 14 View

[Ansys Meshing] [CFD Fluent] Match control and inflation layer

Hi everyone, for my master's thesis I'm simulating the flow around blades of a steam turbine. I want to generate an unstructured mesh with an inflation layer at the walls (for k-omega SST model)....

01 January 1970 9,174 1 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

No title

02 March 2021 3,060 3 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View