History/experience of technology / computing, who wants to participate?

i will write a book on the technological revolutions these last 40 years from a personal experience. who would like to join me and write a chapter?

18 January 2021 3,679 2 View

How would you scale intra-day returns to daily returns?

16 December 2020 6,284 3 View

Looking for help with identification of two MS-spectra of peaks detected in GCMS multiresidue pesticides analyses?

Dear colleagues, I am looking for some support in the identification of the MS-spectra of two unknown peaks detected in a GCMS multiresidue pesticides analyses of an extract of a water sample...

04 September 2020 3,137 0 View

Is there a tool to look for known or simply conserved promoter regions for non-coding RNA?

Predicting promoter regions for ncRNA in bacteria. I am looking for a promoter of a certain RNA I know the sequence and locus of.

01 September 2014 2,993 5 View

How can you find general similarities of enriched mRNAs obtained from single protein immunoprecipitation?

I discovered a few hundred mRNAs that are co-immunoprecipitated with my protein of interest using RIP-Seq. Now I want to find similarities based on sequence, structure, motifs etc. which could...

04 April 2013 5,382 2 View

miRNome and NGS?

I am planning to study the miRNomes in different diseases. Does anyone have experience with the analysis of miRNomes using NGS, especially when starting with a low amount (2-10 ng) of enriched...

26 August 2012 8,854 14 View

[Ansys Meshing] [CFD Fluent] Match control and inflation layer

Hi everyone, for my master's thesis I'm simulating the flow around blades of a steam turbine. I want to generate an unstructured mesh with an inflation layer at the walls (for k-omega SST model)....

01 January 1970 9,174 1 View

Survey on HCI/Human Factors Research Efforts

As we all know, conducting high quality HCI/Human Factors research requires extensive efforts. With the aim to investigate those efforts and later provide support, we are now conducting a small...

01 January 1970 6,291 13 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

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03 March 2021 5,360 2 View

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02 March 2021 3,060 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View