Dear Mahshid Oladi,

Thank you so much for your answer, valuable comments, and the additional information.

I used Spin-Column Extraction Method, and no my DNA template is not visibly pigmented but my original samples (mosquitoes) were heavily pigmented.

I will try this way and feed you back.

Best wishes

I succeeded by adding 1ul of DTT (Dithiothreitol) in the extraction step together with the buffer, before sample maceration. The next purification steps follow normally.

Dear Sirlei Antunes Morais,

Thank you for providing me with this alternative, I really needed it as the previously suggestion did not work well for me.

I will try this with my additional samples, hopefully it might work.

Best wishes

Ayman

Okay Dr Ayman, you're welcome, good luck

Best

Sirlei