I have grown Arabidopsis in liquid MS for 10 days then I transferred them into 5 micromolar coelenterazine for overnight and I add Jasmonic acid isoleucine for 10 mins. now I want to rescue this plant... so tried to transfer them back to same liquid media, MS agar plates also on the soil. but every time these plants don't grow.. I am fed up of trying to rescue them.. can anyone suggest me something to rescue them??
Maybe you could put them on 0.5% Agar plates. What happens when you transfer them back? Do they die immediately or soon after? Make sure that the light conditions are appropriate for growth. By 2 weeks you should be able to move them to soil if they have true leaves, but keep a hood on the flat for a few days to keep the humidity level high. I don't know if this will help.
Thank you Lisa for your answer..
At starting they were looking like they are alive. but not growing. After a week they get whitish and soon die,
Can you rinse them with water after the JA treatment and then transplant them to very moist soil with a dome over their tray? Turning white seems odd, like the chloroplasts are damaged. I don't know exactly. How old are the seedlings when you do the treatment? Just 10 days old? Maybe you could move them to a magenta box since they are so fragile? Sorry I can't help more.
After cleaning, you can also transfer them to a media of perlite and vermiculite and feed them with hoagland solution. This also wash any remaining of chemicals and gradually make them to continue growth normally
Arabidopsis seeds germinate very well in soil. Even seeds grow on agar plates and plantlets with true leaves are later transplated into soil in pots taking care they remain under high humdity by covering them with polythene bags initially. It would be interesting to know why you need to grow these plants in liquid medium.
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