19 March 2014 1 9K Report

What should I do? I am working as a research scholar at an Indian Institute but they do not have such equipment. I want to test the plasma retained from blood for presence of several proteins. Any idea how to go about that?

More Kaalindi Misra's questions See All
I aim to create a co-culture model to see the interaction between T lymphocytes and malignant cells, specifically targeting OSCC,is it possible?

Kindly guide me if any suitable protocols are there to study the interaction

29 April 2024 4,228 3 View

Is there any research group or lab that works on Swarm Intelligence?

Being a researcher in the field of swarm intelligence for more than seven years, I still haven't found any research group/ institution or research lab dedicated to swarm intelligence research....

26 April 2024 4,415 2 View

Is it feasible to co-culture TCR positive macrophages with myeloid cells? If yes, could someone provide a protocol detailing how to conduct this?

TCR is tumor cell receptor positive macrophages which induces inflammation in cancer cell.

07 December 2023 7,299 2 View

What is the formula for commercial beet sugars and invert sugars in sugar beet?

For analysis of sugar beet juice quality, what formula is used to calculate commercial beet sugars and invert sugars

27 November 2023 5,721 0 View

How can we effectively isolate Circulating Tumor cell from whole blood?

CTC( Circulating tumour cells) are defined as tumour cells and at the time of metastasis, these specialised cells come into the circulation which can help to detect the cancer. Kindly provide me...

23 November 2023 2,471 2 View

Impact of Startup success or Failure ?

How much impact of a startup failure comes on Its employee's financial and mental health.

29 August 2023 3,592 0 View

What are the computer vison problems yet to efficiently solve up to 2023?

Is there any list or work or survey on current difficult or hard Computer Vison problems which are yet to solve efficiently?

12 August 2023 5,276 2 View

How do i remove publications that are not mine on research gate but someone with the same name?

i have publications listed that are not mine but someone with the same name

17 April 2023 5,515 1 View

How to make a standard curve for proline estimation in sugar beet?

I have estimated proline in sugar beet leaves and have observed the absorbance at 520 nm. Now I want to make a standard curve for further estimation and calculation of proline. Can anyone help me...

01 June 2022 8,482 7 View

How to add inlets to PDMS fluidics device?

I'm designing a PDMS fluidics device that has millimeter-wide channels (~1-2mm wide), and I'd like to put inlets on it. The PDMS layer is 5-6 mm thick and is plasma bonded to glass. So far, I've...

24 July 2021 8,805 3 View

Similar questions and discussions
Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close