How to remove Trt protecting group from cysteine?

17 November 2022 0 1K Report

I have to cleave Trt group from the thiol of the cysteine: I kept the reaction to stir in TFA:TIS:H2O 95:2.5:2.5 (5 mL for 0.7 mmol) for 2 hours, but the reaction didn't go to completion (around 50-50 of protected:deprotected). Do you have suggestions?

Badges
Science topic

More Kelly Bugatti's questions See All

Is there a diagnostic difference between pathological demand avoidance and ASD? Why a surge of preschoolers refusing to engage in learning activities?

I understand that Pathological Demand Avoidance or PDA is not a diagnosable condition listed in the DSMV but it is gaining notoriety in the field of special education and with ASD researchers....

20 April 2024 3,858 2 View

Which selective media can I use to isolate entomopathogenic microorganisms?

Most entomopathogens that exist on insects and in nature are always in association with a number of other microbes. I strongly feel other ubiquitous microbes have a faster regeneration time...

25 March 2024 3,927 1 View

Unsoluble brown residue during Ulmann-type reaction?

I performed an Ulmann type reaction bewteen 2-pyridone and bromobenzene, with these reaction conditions in a schlenk tube: - 0.3 eq CuI - 2 eq of K2CO3 - 1 eq of pyridone - 1.1 eq of...

10 March 2024 3,916 2 View

What is the best measurement of organizational trust?

I want to measure the level of organizational trust for a leadership study. Which would you recommend?

14 November 2023 513 5 View

What are the best ways to measure stress - biometric or otherwise - for a leadership study?

Hi all, I am thinking of collecting data on stress for a leadership study I am doing. For HRV, I was thinking to use wearable devices. But I am also thinking of cortisol strips. Any other good...

17 September 2023 4,969 3 View

Retinitis pigmentosa study proposal?

Would anyone like to do a before and after visual field study on me and a few others with RP? The Wellspring Clinic in Vancouver Canada is helping a lot of people that I know via accupuncture i...

11 September 2023 4,047 0 View

PCA with Panel Data?

I would like to construct principal components from 30+ financial ratios for a predictive model. I would then use logistic regression and support vector machines for the predictions, conditioned...

29 August 2023 7,714 4 View

Can you recommend an inexpensive options for a microscope with camera to image seagrass flowers/pollen and zooplankton? Would UV light help?

Flower and pollen size is highly variable as well as the size of the zooplankton.

21 June 2023 5,071 1 View

Is there an easy way have snapgene select compatible restriction enzymes that delete a sequence from my plasmid?

I am using a gateway cloning vector to overexpress a gene, but I want a control vector as well (without my insert). I want to cut out the ccdb gene so it wont self destruct but I dont have a...

08 June 2023 6,241 2 View

Highly recommended papers on psychology's OCEAN five factor model?

Seeking highly recommended papers on psychology's OCEAN five factor model of human behavioural traits. Who are the best authors in this field, and can you recommend any high quality papers...

04 June 2023 4,236 0 View

What plants have high levels of sulfur-containing amino acids such as methionine?

Methionine, cysteine, homocysteine, and taurine are the 4 common sulfur-containing amino acids, but only the first 2 are incorporated into proteins. Sulfur belongs to the same group in the...

22 July 2024 4,273 4 View

Would it be possible to determine how many Cys I have based on Ellman's assay?

I want to predict the DAR ratio based on the number of cys available for conjugation to Cys residue. I use Ellman's assay to predict the concentration of Cys after TCEP reduction. I gain a...

02 July 2024 612 6 View

Media Preparation protocol for Bifidobacteria Agar and Broth?

Hello everyone! I have been trying to develop a protocol for my thesis research which includes culturing and storing Bifidobacteria. I have come across many articles and studies related to the...

10 June 2024 4,152 1 View

Hi, How can I design primers to obtain Deletion mutants by Site Directed Mutagenesis?

I have done site-directed mutagenesis (Substitution) to obtain a Cysteine mutation in my gene of interest. Now, I have two domains in my protein, And I want to delete one of the domains to obtain...

05 June 2024 9,430 1 View

Antibody maleimide conjugation - TCEP method affected by tris concentration in purified buffer?

As title describes, we're trying to mutate an amino acid of antibody Fc to cysteine, followed by TCEP to further drug conjugation, one of my colleague had raise the concern of purified TCEP...

29 April 2024 3,227 2 View

Free thiol in structure, how to proceed for amide coupling?

I'm trying to do a coupling reaction between a carboxylic acid and an amine. However, my acid has a free thiol group in the structure, which I cannot remove. Is there any selective coupling agent...

14 February 2024 5,964 0 View

Disulfide polymer reduction with TCEP and DTT precipitation?

I'm working on a polymer with NIPAAm and n,n-bis(acryloyl)cystamine ( molar ratio ~ 15:1 ), and I want to reduce the disulfide into thiol group. I used TCEP dissolved in PBS and adjust the pH ~ 7,...

21 January 2024 1,704 0 View

How to do MD simulation of protein with disulfide bond?

Hello! I'm trying to simulate AKT1 protein in water, since this protein structure has a disulfide bond between two cysteine residues and some missing residues so I have to model the sequence with...

14 January 2024 5,906 1 View

How to keep L-cysteine from converting to cystine?

How to keep L-cysteine from converting to cystine? Even at low pH. Are there any reducing agent may work? better FDA allowed

25 December 2023 4,383 2 View

Looking for help regarding thiol-ene or azide-alkyne click reaction?

Recently, I am working on a project in which i need to attach an aptamer with the COF. The COF has a functional group (ene or yne) and the aptamer has thiol or N3 group. However, due to some...

19 December 2023 1,614 0 View