Kelly Bugatti

8 Questions 6 Answers 0 Followers

Questions related from Kelly Bugatti

Unsoluble brown residue during Ulmann-type reaction?

I performed an Ulmann type reaction bewteen 2-pyridone and bromobenzene, with these reaction conditions in a schlenk tube: - 0.3 eq CuI - 2 eq of K2CO3 - 1 eq of pyridone - 1.1 eq of...

11 March 2024 3,963 2 View

Maleimide Thiamichael: is it selective for Cys in a peptidic structure?

I need to click a peptide (8 aminoacids) to a probe functionalized with maleimide moiety. The peptide contains a Lys, a terminal NH2 Leu and a Cys. What are the condition to make the reaction...

13 January 2023 8,707 2 View

How to remove Trt protecting group from cysteine?

I have to cleave Trt group from the thiol of the cysteine: I kept the reaction to stir in TFA:TIS:H2O 95:2.5:2.5 (5 mL for 0.7 mmol) for 2 hours, but the reaction didn't go to completion (around...

17 November 2022 1,144 0 View

How can I avoid the Boc-cleavage during Mass Analysis?

I work with peptide/peptidomimetics (1600-1800 Da) and when I perform Mass Analysis (flow injection, ACQUITY Ultra Performance LC; ESI, positive ions, Single Quadrupole analyzer) I often find...

16 July 2021 5,189 4 View

Is it possible to have HOAt as impurity after the cleavage from the resin (in SPPS)?

I'm used to synthesize peptide using the Fmoc-based Solid Phase Peptide Synthesis, with HATU/HOAt as coupling reagents. This time, after the cleavage from the 2Cl-Trt resin, I see the peaks of...

27 May 2021 7,624 1 View

How to cleave terminal amine from 2-Chlorotrityl Resin?

I have to cleave a terminal primary amine from the 2-Chlorotrityl Resin and I have to mantain the rest of the protected peptide (Boc/tBu protecting groups). I usually use a solution of 3/1/1...

05 May 2021 9,281 4 View

Stability of N-Benzyl substituted indazoles?

I am working with N-Benzyl substituted indazole-nucleus but I'm having many problem with the stability of the molecules. Does anyone know if the benzyl position is sensitive to something, such as...

30 June 2020 7,713 3 View

How can I purify two different-substituted aromatic compounds?

I did the reaction shown in the image attached to this question, reported in the article with DOI: 10.1021/jm900431g I need the first product (the ortho-nitro substituted one), but I have always...

19 January 2019 7,350 5 View