Kelly Bugatti

4 Questions 2 Answers 0 Followers

Questions related from Kelly Bugatti

Maleimide Thiamichael: is it selective for Cys in a peptidic structure?

I need to click a peptide (8 aminoacids) to a probe functionalized with maleimide moiety. The peptide contains a Lys, a terminal NH2 Leu and a Cys. What are the condition to make the reaction...

13 January 2023 8,637 2 View

How can I avoid the Boc-cleavage during Mass Analysis?

I work with peptide/peptidomimetics (1600-1800 Da) and when I perform Mass Analysis (flow injection, ACQUITY Ultra Performance LC; ESI, positive ions, Single Quadrupole analyzer) I often find...

16 July 2021 5,129 4 View

Is it possible to have HOAt as impurity after the cleavage from the resin (in SPPS)?

I'm used to synthesize peptide using the Fmoc-based Solid Phase Peptide Synthesis, with HATU/HOAt as coupling reagents. This time, after the cleavage from the 2Cl-Trt resin, I see the peaks of...

27 May 2021 7,563 1 View

Stability of N-Benzyl substituted indazoles?

I am working with N-Benzyl substituted indazole-nucleus but I'm having many problem with the stability of the molecules. Does anyone know if the benzyl position is sensitive to something, such as...

30 June 2020 7,614 3 View