Are there any recommendations on how to safely remove the chamber from a chamber slide used for 3D cell culture (using reconstituted basement membrane matrices)? I am concerned primarily with shearing and/or distortion of the gel as the chamber is being dislodged, any tips/suggestions will be highly appreciated, thanks!
The chamber slides I've used previously with a specific tool for removing the chambers, that slides over them and breaks them free. If you can't find this tool or didn't get it, you can try using a sterile razor or scalpel along the points where the bottom of the chamber meets the slide.
I have used the chamber slides from Nunc.
https://www.sigmaaldrich.com/catalog/product/sigma/c6932?lang=de®ion=DE&gclid=Cj0KCQiA962BBhCzARIsAIpWEL2NA9a8y8j8CYdRdZfZU8W_9KkioDujGEipqBlToz5oK5ezlVNbfmYaAl5LEALw_wcB
You can remove the side walls easily with the applicator that comes with the box. Just keep in mind. You must be very careful and gentle while inserting the tool and lifting the plastic chamber. I always tried to have the gels in the middle of the well so that they won't attach to the sidewalls and then I have an easy process.
In the end, it is a matter of training.
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