How to remove the bacterial contamination from yeast (Sacharomyces cerevisiae or other species of Sacharomyces) culture?
On plate cultures you can add 50mg/L chloramphenicol to your media (e.g YPD, WL, etc) to eliminate bacteria. This also works in liquid cultures. for Saccharomyces you can try using DRBC (Dichloran Rose Bengal Chloramphenicol Agar) as and easy preparation and eliminate handling antibiotics directly.
If you don't want to add antibiotics then you can try decreasing pH and incubating anaerobically if you think the bacteria maybe just contamination from the environment
Recommend that you use serial dilutions to get separated and distinct single colonies. Grow these out sufficient time to observe and discard any with have any traces of contamination. In your dilution you might consider using surface disinfection technique. The media can be acidified with lactic acid playing with these factors you should be able to get clean cultures.
Add 50 mg/L chloramphenicol in your growth medium to get rid of bacterial contamination. Also, you may acidify the growth medium (pH 5.5-6).
Dear Dr.Amin,
We are working in the field of Medical and Veterinary Mycology since 1973.
Please prepare serial dilution of the isolate grown in plate, and then further inoculate on any media , such as Sabouraud medium, potato dextrose agar, Pal sunflower seed medium (1980) and APRM agar (2015), which must contain chloramphenicol (100 mg/L, pH 5.5) to suppress the growth of bacteria. Then you should prepare wet mounts of a single isolated colony either in PHOL stain or Narayan stain to study the morphology. This will help you that the culture is pure and not contaminated with bacteria. In addition, I advise you to please use Laminar flow for the isolation of the microbes from clinical and environmental materials.
We developed PHO stain and Narayan stain in 1990 and 1998, respectively. All our publications on Pal sunflower seed agar, APRM agar, PHOL stain, and Narayan stain can be easily downloaded from Research Gate or Academia.
Stay safe and healthy. With best wishes and good luck, Prof.Dr.Mahendra Pal Founder Director of Narayan Consultancy on Veterinary Public Health and Microbiology
- Badges
- Science topic
- Similar topics
- Physical Sciences
- Materials Science
- Plating