How to remove lipids from serum in order to purify an enzyme from serum?

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Baiba Kurins

The method developed by Cham and Knowles (1976) J. Lipid Res 17, 176-181 works very well to remove the majority of serum or plasma lipids. We adapted this method for a microscale purification of gangliosides: Ladisch and Gillard (1985) Anal. Biochem. 146, 220-231. Of note, our partition method leaves about half of the GM3 ganglioside in the upper organic phase.

Charles D Anderson

Phase extraction with chloroform