Need chlorophyll free sample to estimate enzyme concentration but unable to remove trace amount of chlorophyll which impairing the result..!
You may try to use GCB (Graphitized Carbon Black) and or PSA (Primary Secondary Amine) to remove chlorophyll and other pigment also.
Please check these researchgate links.
https://www.researchgate.net/post/How_can_I_remove_chlorophyll_from_leaf_extract
https://www.researchgate.net/post/How_can_I_remove_chlorophyll_from_leaf_extract_without_losing_other_secondary_metabolites
https://www.researchgate.net/post/How_can_I_remove_chlorophyll_from_plant_extract
you can participate the protein solution with cold acetone HPLC grade three times until the obtained protein solution is clear without any pigments. please carryout all steps under cooling conditions.
Hi Dear
I hope you are doing well, please let me have recipe for remove pigment from enzyme buffer.
Best regards
maryam
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