How to reduce the erucic acid in canola seeds in tropical and sub-tropical region?

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Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Trying to make optimization for Mixer on different pdk ?

i make a design topology of mixer For my graduation project working at 28 Ghz on cadence with UMC pdk and get this result S(2,1) 3.5db but when trying to make same topology with another pdk on...

09 June 2024 9,729 3 View

Is it good choice to use the SARC/J auxiliary basis set with def2-SVPD basis set without including any relativistic effects like ZORA or whatever?

I’m currently utilizing the ORCA software for my computations, applying the PBE0 functional combined with the def2-SVPD basis set and incorporating the RIJCOSX approximation. For the auxiliary...

07 June 2024 6,352 0 View

Do the methods of calculating the absorption coefficient (Alpha) from absorbance differ between powder samples and thin films?how is it calculated ?

When using the absorption spectrum to determine the absorption coefficient (Alpha), do the calculation methods differ between powder samples and thin films? If there are differences, what are the...

07 June 2024 8,797 2 View

How do quick initial-test to select antibiotic-producing fungal strains?

We have dozens of pure fungal strains recovered from the soil and need to know the extent of their ability to produce antibiotics. Still, the problem is the spread of fungal mycelia on the plate...

09 May 2024 9,467 2 View

Does anyone have detailed methods and instructions on how to count the numbers of intraepidermal nerve fibers (IENFs)/mm using Fiji - ImgaeJ?

Counting the numbers of intraepidermal nerve fibers

05 May 2024 3,296 1 View

Effects of Aeromonas caviae and Aeromonas veronii on Freshwater Fish?

28 April 2024 9,191 1 View

Convert solid organic powder to nanoparticle?

i synthesized schiff base with bulk size., any suitable method to convert it in nanosize with full regards

22 April 2024 1,423 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

Posthoc test lettering in JAMOVI?

Does anyone know of a module for the JAMOVI software that is capable of generating mean separations using the classic letters based on post hoc results (e.g., Tukey test)? If, as I believe, such...

31 July 2024 3,333 4 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

29 July 2024 4,570 0 View

My cfDNA extraction protocol using silica beads works with plasma collected in COD SAGM bags but not with plasma collected in K2 EDTA tubes. Why so?

I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...

28 July 2024 3,559 0 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

How can I use DFT-D3 correction ( Siesta version 5.0.1)?

Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...

27 July 2024 5,748 0 View