Tissues getting smudging ,Shrinkage when the cryo-section brings to room temperature...Due to OCT surface tension...Problem persist even after immediate fixation...
According to my recent literature data analysis in connection with our thermodynamics transformation theory, the unfolding of the secondary structure of polymers (peptides) having helical conformations can be controlled by reducing its surface free energy by exposing them to the neutral and basic aqua solutions-deionized- water containing 0.1M NaCl plus concentrated solution of of NaOH for pH adjustment (having high pH levels equal and greater than 5-6 pH levels,) which can reduce their helicity drastically that causes formation of crossed linked chain structures. On the other had pH level equal and less than 4 pH causes formation of alpha-helical conformation, which can be obtained by using HCl concentrated solutions to adjust the pH levels. (See following paper)
A. Idıris et. al. ''Spring mechanics of alpha-helical peptide'' Protein Engineering vol.13 (11)pp.763-770 (2000).
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