How to search MS spectrum to identify the compounds?

With the active fractions from Si-gel CC, I did high resolution LC-MS/MS experiments and got the spectrum. However, I haven't find any reference of purifying the compounds from the same sample....

05 June 2018 2,558 6 View

Can anyone help me to learn molecular dynamics calculation of ligand-protein docked conformation?

I am interested in Molecular dynamics calculation. After finishing the docking, I would like to carry out dynamics calculation to understand the stability of the ligand-protein pose. If anyone can...

03 April 2018 1,078 4 View

How to search AIST Database with chemical shifts?

Can anyone tell me how to search AIST database with proton/carbon NMR chemical shifts? Before I tried, but in the long run failed to get the suggested chemical structure. Your cooperation would...

09 October 2017 3,282 3 View

Which method should I follow next to purify the active natural products?

I have collected one active fraction from Si-gel CC using Hexane:EtOAc gradient. The active fraction eluted with 3:2 ratio. Now its time to purify the active compounds from this fraction. TLC...

08 September 2017 9,167 7 View

How to elucidate the structure using NMR chemical shifts?

I would like to elucidate chemical structure of an unknown sample using proton-NMR, Carbon-NMR, 2D-NMR and Mass spectrum Data. Can anybody suggest me how to do this and what type of references I...

08 September 2017 5,762 10 View

How to get the pure acid from dihydrated sodium salt of an acid?

07 August 2017 5,671 6 View

Can anyone tell me the reaction in details?

06 July 2017 4,585 2 View

Can I use well plate directly for staining of the cells?

I would like to stain the cells with DAPI. I am using 24 well plate for cell culture. Is it possible to stain and observe under fluorescence microscope using the well plate directly? Could anyone...

31 December 2016 6,404 5 View

How to isolate specific peak (which represent bioactive compound) using preperative HPLC?

I would like to isolate more polar compound from the extract. In this regard, I am supposed to use HPLC for successful isolation of the compound (s). Can you please suggest me the procedure for...

07 August 2016 2,911 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

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02 March 2021 3,060 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View