Md. Shahinozzaman

10 Questions 6 Answers 0 Followers

Questions related from Md. Shahinozzaman

How to search MS spectrum to identify the compounds?

With the active fractions from Si-gel CC, I did high resolution LC-MS/MS experiments and got the spectrum. However, I haven't find any reference of purifying the compounds from the same sample....

06 June 2018 2,630 6 View

Can anyone help me to learn molecular dynamics calculation of ligand-protein docked conformation?

I am interested in Molecular dynamics calculation. After finishing the docking, I would like to carry out dynamics calculation to understand the stability of the ligand-protein pose. If anyone can...

04 April 2018 1,156 4 View

How to search AIST Database with chemical shifts?

Can anyone tell me how to search AIST database with proton/carbon NMR chemical shifts? Before I tried, but in the long run failed to get the suggested chemical structure. Your cooperation would...

10 October 2017 3,351 3 View

Which method should I follow next to purify the active natural products?

I have collected one active fraction from Si-gel CC using Hexane:EtOAc gradient. The active fraction eluted with 3:2 ratio. Now its time to purify the active compounds from this fraction. TLC...

09 September 2017 9,236 7 View

How to elucidate the structure using NMR chemical shifts?

I would like to elucidate chemical structure of an unknown sample using proton-NMR, Carbon-NMR, 2D-NMR and Mass spectrum Data. Can anybody suggest me how to do this and what type of references I...

09 September 2017 5,834 10 View

How to get the pure acid from dihydrated sodium salt of an acid?

I would like to prepare Tolmetin from Tolmetin sodium salt dihydrate (TSSD). TSSD is an commercial product, however I would like to work with Tolmetin only.

08 August 2017 5,767 6 View

Can anyone tell me the reaction in details?

I would like to know the details about the reaction attached. It would be highly acknowledged in any one can explain it to me. Thanks in advance.

07 July 2017 4,648 2 View

Can I use well plate directly for staining of the cells?

I would like to stain the cells with DAPI. I am using 24 well plate for cell culture. Is it possible to stain and observe under fluorescence microscope using the well plate directly? Could anyone...

01 January 2017 6,455 5 View

How to isolate specific peak (which represent bioactive compound) using preperative HPLC?

I would like to isolate more polar compound from the extract. In this regard, I am supposed to use HPLC for successful isolation of the compound (s). Can you please suggest me the procedure for...

08 August 2016 2,988 4 View

How to reduce DMSO concentration in cell culture based assay?

I use different cancer cells to expound Bioactivites of the isolated/target compounds. I dissolve the compounds in 100% DMSO, and I also use 24-well plate. In each well, I put 1 mL cell...

07 July 2016 8,101 1 View