please help me
I'm studying about soft lithography. If I'm not mistaken, the process is almost the same with NIL. But the difference is NIL uses hard mask, and soft lithography uses a mold made of HMDS. My...
15 March 2024 2,960 0 View
How to write Spectroscopic Notations for organic molecules?
19 November 2023 6,940 2 View
I have an exoenrate output file with gff2 format for protein seq alignment. I want to convert this output file into a gff3 format. How can I do this?
07 October 2023 932 1 View
Hi all, Initially TCNQ gave well defined 2 electron reduction peak. After addition of polymer to it, I could able to see one more peak that was absent in the background( TCNQ+TBAP).
23 September 2023 2,799 0 View
I'm currently annotating a plant genome. I already identified repeats in the genome using LTRStruct. I need to supplement the resulted file using Repbase data for repeat masking step. Which file...
07 July 2023 7,510 0 View
Currently I'm working on a plant genome annotation work. For my plant, I don't have any RNA sequences or ESTs. Also very little ESTs and RNA sequence data from closely related species. Is it okay...
26 June 2023 3,509 2 View
I need to download RNA sequence data from OneKP project. I know the plant accession code for the plant I'm looking for. But there are multiple files per plant accession. Which one do I need to...
26 June 2023 1,433 1 View
Let me know your thoughts
13 June 2021 1,639 4 View
Does this include spring-dashpot elements?
04 March 2021 1,334 2 View
Materials science journals
09 July 2020 4,990 5 View
Is this circle one of the rings?
03 August 2024 5,987 0 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,541 11 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...
28 July 2024 8,025 3 View
or can you recommend articles showing the differential effect of LPS in a time dependant manner?
28 July 2024 2,547 1 View
So - my lab did a bunch of RNA isolations from tissue culture cells, for purposes of RNA-seq, and submitted them to a core facility. We got back a notification that the RINs were too low, and we...
14 July 2024 641 1 View
Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...
13 July 2024 1,674 3 View
I want to make some yeast extractions with a protocol that uses Isopropanol and NaOH. So in the end i will have an azeotrope of Isopropanol and Water that comes from the cell and NaOH. Can you...
08 July 2024 9,201 2 View
Hello everyone, I'm in the initial stages of conducting a study involving the ddPCR (Bio-Rad QX200, QX600) and I ran into some problems involving recovery % calculations using direct...
02 July 2024 6,011 2 View
