Hi,
I acidentally diluted my genomic DNA into too much TRIS HCl buffer (10x too much). I want to recover the concentrated form of genomic DNA.
Is there any kit or column where I can do this?
Hello Abel,
maybe you could try to precipitate your DNA using isopropanol and sodium acetate ?
Hi, Abel Tan
Precipitate the DNA by adding 0.7 volume cold isopropanol and incubate at -20°C for 15 minutes
Centrifuge the sample at 14,000 x g for 10 minutes. Decant the supernatant without disturbing the pellet and subsequently wash with 500 µl ice cold 70% ethanol.
Decant the ethanol. Remove residual ethanol by drying in a SpeedVac
put it in a pcr machine set at 70c and evaporate the liquid if you do not have a speed vac.
you dont tell us how much DNA you are working with.
If a few micrograms, I would add 1mg of glycogen (1uL) to produce a visible pellet after precipitation with 0.1 volumes of 3M sodium acetate and 2 volumes of ethanol. but thats only if you know that glycogen isnt going to affect your downstream application.
Pauls suggestion of evaporation will work (dont cap the tube or use the heated lid!!), but of course you will end up with DNA in 10x the buffer that you added by mistake. Maybe that's not a problem?
The easiest solution is to evaporate some of the water from your buffer. The problem with that is that it would clearly increase the salt concentration in your sample - so don't "over dry" the sample. The way to go is to use a speed vac machine or to heat with an open lid.
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