Does anyone have experience with TGFbeta reconstitution? I want to reconstitute it but the final conditions of the solution should be compatible with my SPR running buffer, which is PBS-P+3%DMSO. I was thinking of reconstituting it in PBS-P 4mMHCl containing an appropriate amount of DMSO. Do you think the buffer (PBS instead of water) will affect the dilution (by affecting the final pH for example)? Will DMSO help? Why use HCl to reconstitute TGFbeta, where does it help?

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