Anastasia Patsiarika

6 Questions 6 Answers 0 Followers

Questions related from Anastasia Patsiarika

Does anyone have a protocol for growing Rosetta gami 2 in minimal media?

I would like to know how I should supplement the minimal media because my cells are not growing

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Inconsistent MST trace?

I'm using MST to investigate protein-protein inteactions. Everything seems to be set up properly, no negative outcomes in the binding check. However, I keep on getting inconsistent traces from the...

10 October 2019 6,284 3 View

Anyone using scraping to harvest RAW264.7 cells and has problem with flow cytometry?

So I am about to use Raw264.7 cell line in flow cytometry to measure the signal of an intracellular protein. The cells are know not to effectively detach with trypsin so I will use scraping. Will...

06 June 2019 3,967 5 View

SPR Yes/No binding of protein-protein interactions?

I try to understand if two proteins interact by using Biacore T200. The ligand is 35kDa and the analyte 24kDa. At an immobilization level of 2000RU of the ligand I get a signal of 400RU of the...

30 April 2019 4,478 3 View

How to reconstitute TGFbeta for SPR?

Does anyone have experience with TGFbeta reconstitution? I want to reconstitute it but the final conditions of the solution should be compatible with my SPR running buffer, which is PBS-P+3%DMSO....

11 April 2019 486 0 View

What parameters should I use in Biacore T200 kinetics/affinity?

So I am using Biacore T200 and want to perform a kinetics analysis on a small molecule that is bound to my protein of interest. My question is what parameters should I use, for example...

04 April 2019 3,048 1 View