Anastasia Patsiarika

7 Questions 7 Answers 0 Followers

Questions related from Anastasia Patsiarika

Does anyone have a protocol for growing Rosetta gami 2 in minimal media?

I would like to know how I should supplement the minimal media because my cells are not growing

10 April 2021 8,594 3 View

Inconsistent MST trace?

I'm using MST to investigate protein-protein inteactions. Everything seems to be set up properly, no negative outcomes in the binding check. However, I keep on getting inconsistent traces from the...

10 October 2019 6,323 3 View

Anyone using scraping to harvest RAW264.7 cells and has problem with flow cytometry?

So I am about to use Raw264.7 cell line in flow cytometry to measure the signal of an intracellular protein. The cells are know not to effectively detach with trypsin so I will use scraping. Will...

06 June 2019 3,998 5 View

SPR Yes/No binding of protein-protein interactions?

I try to understand if two proteins interact by using Biacore T200. The ligand is 35kDa and the analyte 24kDa. At an immobilization level of 2000RU of the ligand I get a signal of 400RU of the...

30 April 2019 4,504 3 View

How to reconstitute TGFbeta for SPR?

Does anyone have experience with TGFbeta reconstitution? I want to reconstitute it but the final conditions of the solution should be compatible with my SPR running buffer, which is PBS-P+3%DMSO....

11 April 2019 523 0 View

What parameters should I use in Biacore T200 kinetics/affinity?

So I am using Biacore T200 and want to perform a kinetics analysis on a small molecule that is bound to my protein of interest. My question is what parameters should I use, for example...

04 April 2019 3,087 1 View

Virtual screening strategy?

I am using Gold to dock the Maybridge fragment library into the PDE4b target. I use Chemscore for docking and ASP for rescoring (as suggested by the program template for phosphodiesterases). I...

02 February 2019 3,940 5 View