Are cloud points able to identify tree species?

Hello Dears My goal is to classify dense forest tree species using drone images. Is it possible to classify dense cloud points? If yes, in what way and in what software? Thankful

15 February 2021 3,475 3 View

Do you have any information on modeling the diameter growth of conifers?

Hello dear friends Regarding modeling the growth diameter and cross section of conifers, I need a number of articles that have researched in this field. have you any recommendation? Thank you for...

20 January 2021 6,532 4 View

What is the classification of tree species by texture analysis technique on drone images?

Hi My goal is to distinguish forest tree species (in dense forests) with drone images. I did not get acceptable results using spectral features. Because the pictures were taken in the fall and the...

07 January 2021 7,494 3 View

How to classify a base object with drone image?

Hello beloveds. I am classifying dense deciduous trees with eCognition software. In fact, it is the separation of tree species with drone images. The image has a near infrared band, red, red...

29 December 2020 5,900 2 View

What is the best flight altitude for a drone in the forest?

My goal is to use the short-range remote sensing technique (drone) to study tree species in deciduous forests. Overlap and flight altitude play a key role in this. Do you have the right overlap...

02 December 2020 2,442 4 View

What are new trends in heat transfer and fluid mechanics?

Hello I'm currently searching for new trends related to heat transfer and fluid mechanics to select my M.Sc. thesis topic. can you please introduce me innovations and recent advancements in this area?

30 November 2020 9,316 13 View

What is the best method of discriminant data analysis?

I have three dataset of quantitative and qualitative liquid chromatography, GC-MS and DNA barcoding of few samples. What would be the best way to discriminate and visualize the data?

25 October 2020 1,173 9 View

Does anyone know what is an appropriate statistical analysis of drug release studies?

I am measuring the drug release of a few drug-eluting implants in dissolution medium in different time intervals. All the measurements are in triplicates. Would ANOVA or TTEST is an appropriate...

20 September 2020 8,183 5 View

When should I harvest the yeast cell growing in galactose medium to perform qPCR?

Hi, I engineered the yeast Saccharomyces cerevisiae to over-express some of its native genes, in addition to native copy of gene. For overexpression, I used strong modified GAL1 promoter ( that...

12 November 2018 496 3 View

Is there any enyzem/protein that can target a short DNA sequence (not promoter), and this complex can then behave as a regulator?

I am searching for a protein (or transcription factor) that can interact with a short DNA sequence (that is NOT promoter or some part of promoter for another gene) and this interaction results in...

18 April 2018 4,917 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

How to approach CFD simulation results of a flow past a cylinder?

Hi all, I'm doing a transient simulation of flow past a rectangular cylinder close to the free surface. As far as I'm concerned, the common practice is to discard the initial stages of the...

25 February 2021 1,348 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How can I selectively dry etch Ti on a polymer substrate?

24 February 2021 5,378 1 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View