My Huh-7 cells get vacoulized in passage 85 and I dont know why. Is there anybody to have the same experience? please help me.
Is it acceptable to include secondary literature, such as other scoping reviews, umbrella reviews, meta-analyses, or systematic reviews, in the process of conducting a scoping review?
29 May 2024 8,975 2 View
to be involved in our research please massage me here
15 May 2024 8,998 2 View
Hello When we pattern a mask in a wafer, after exposure we noticed that the length of our patterns in the center of our wafer is thicker in copmarison with edges of it. What is the reason and...
22 April 2024 6,796 4 View
Hello, I have 2 questions regarding cancer mRNA vaccines. When synthetic mRNA vaccine for cancer is introduced into the body, our expectation is that the immune system will be activated against...
09 March 2024 2,858 2 View
Can someone send me a model of a suspension bridge in Sap2000?
21 January 2024 1,233 0 View
Hi! I need to prepare Ti3C2Tx dispersion in water and through reviewing the relevant papers, I noticed that some researchers have reported Argon as the atmosphere for sonication while others...
28 December 2023 3,496 4 View
Hello. I wanted to have the information of a specific MR damper that is suitable for use in the suspension bridge and connecting to the sub-cables of the bridge. My requested information includes...
22 December 2023 3,080 1 View
Is there an article for dynamic balance test?
18 November 2023 2,460 2 View
Hi everyone im trying to evaporate gold with electron beam evaporation. but haven't shown any adhesion. i have used microscope slides as well as Titanium as a adhesion layer but there haven't...
06 November 2023 333 1 View
Advantages and disadvantages of usage of aqueous solvents in this method.
04 November 2023 2,987 0 View
"Huh7 cells were incubated for 6 h with DEN-1 sNS1 (10 ug/ml) or PBS, followed by 24 h or 48 h of chase (6 h/24 h and 6 h/48 h, respectively)." This statement is from the paper titled "The...
04 October 2023 5,082 1 View
Huh7 cells are more cancerous than HepG2 cells due to the point mutation at p53 tumor suppression gene. And, In HBV expression, HBV can express in Huh7 even at the lack of HBx. (HBV with HBx...
11 July 2023 3,110 0 View
Hi everyone, I'm looking for very adherent cell lines for a couple of experiments. There seems to be little information online. In my experience, HEK < Huh7 < Vero With Vero being...
29 May 2023 1,887 5 View
0.1 million cells in 1 ml media were added per well. Same pattern observed in all 12 wells during multiple platings.
15 May 2023 4,958 3 View
I am going to culture cancer stem cells from HCC cell line Huh7. But I do not know how to set out to do. What kinds of medium should I use? Does it need any growth factor such EGF? Would anyone...
10 February 2023 2,452 4 View
I am currently working with HepG2, HepAD38, Huh7 and HepG2-NTCP-K7 cell lines. I want to know what are the correct timeline for those cell lines.
26 January 2023 5,490 3 View
I am currently trying to add a tagRFP protein to the C-terminal of my target in the Huh7 cell line. The outcome was acceptable. I got more than 40% of cells generating red fluorescent 36 hrs after...
25 October 2022 3,654 3 View
I am trying to make LNP for mRNA delivery using Dlin MC3-DMA,DSPC,cholesterol and DMG-PEG2000 (50:10:38.5:1.5 molar ratio) by rapid mixing method, but I am unable to successfully transfect the...
28 July 2022 577 0 View
Dear folks I am facing a problem with my HUh7 cells grown in DMEM (high glucose, L-glutamine +, sodium pyruvate -) with 10% FBS , Non-essential amino acids (1X), L-Glutamine (2mM), Antibiotic....
31 December 2021 2,152 0 View
I am going to do research on a herbal component and its effects on the liver for hyperlipidemia. Is there any significant difference between them that makes me work on both of them?
28 May 2021 5,961 6 View