We have an antibody that we received as a gift from another lab (not commercially available) and the liquid has evaporated, leaving a sticky substance at the bottom of the tube. Is it possible to add liquid to use the antibody again? If so, what kind?
Hello Merav Gold
Yes, you may add liquid to use the antibody again provided that the antibody is not degraded.
You may use 1X PBS. BSA may be added to the antibody solution as a stabilizer for long-term storage. Adding 0.2 - 1% BSA will help stabilize the antibody solution and generally will not interfere with immunodetection methods.
Best.
you received antibody, was it at 4C or RT. Under what condition, liquid dried off. Try to add the diluent and titrate if it works and store at the proper temperature after making aliquotes and by adding sodium azide as preservative if necessary. Discuss with the person who supplied you antibody in the first place about this. He will be best one to guide you
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