Hello.
We are currently observing the in vivo environment of mice at different glucose levels and
For this reason, we are currently measuring blood glucose levels in mice that have been administered anesthesia beforehand and treated with an open abdomen.
When we perform the laparotomy, we inject 30% glucose through a needle into the stomach of the mice.
However, the blood glucose level did not rise at all even after waiting 30 minutes after administration, and the mice died during the procedure after being warmed up to the 38°C range with a heater.
We are looking for a way to raise the blood glucose level in order to finally observe the glucose uptake in the pancreas, but do you have any good ideas on how to administer it?
Incidentally, we are currently considering direct glucose administration via tail vein, but no one has tried it and we are still in the consideration stage.
Applying 30% Glucose into the abdominal cavity should result in serious osmotic stress, but not in glucose uptake.
For simplicity, you could try to infuse a glucose solution by the tail vein, using a syringe pump (hospital style or homemade, e.g. taking advantage of sth like a Cavro XP-3000 with a small volume syringe, which you might find on the used market. They are quite easy to control from a laptop through a USB-to-serial converter and sending commands from a terminal program).
An alternative injection point might be the jugular vein, but this approach requires some surgery. For an example with rats, please refer to this paper:
Article Prolonged glucose infusion into conscious rats inhibits earl...
Dear Mr. Wolfgang
Thank you for advising some methods. Before buying those syringe, I tried vein injection with 27G needle, include PBS.
As a technical failure, the needle inevitably entered subcutaneously or intradermally when injecting the tail vein in mice, and only twice in dozens of attempts was the needle allowed to enter the tail vein.
I will try a few more challenges such as the angle of the needle and the dilation of the vein, but so far tail vein injections seem to be difficult.
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