Here I have choose one article for learning ImageJ software from that I can calculate the velocity, moving distance etc. I have uploaded the picture from that paper. But here they mentioned about the diameter of the plate. What does that mean? Can anyone help me to quantify touch evoke response in zebrafish larvae by using ImageJ? Thank you.
The output of ImageJ software during measurement of properties like diameter and area will be pixel.
So the above method mentioned was used for the conversion of pixel value to mm (milimeter). As the size of the petridishes are standard or can be measured so that can be used as the reference for the other objects in image.
Not in imageJ but did in matlab and python... The procedure u have mentioned image is just an calibration process and will be same in all..
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