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Questions related from Yuning Hou
Our lab is going to purchase some mouse embryo from EMMA (European Mouse Mutation Archive). In order to fill out the MTA, we need to provide the information of the provider and administrator to...
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I'm trying to ligate a 60bp DNA insert into PLKO.1 vector (7kb) but I met some problems during ligation. The vector was double digested with AgeI and EcoRI and purified from gel. And the DNA...
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I'm trying to collect conditioned media from endothelial progenitor cells (EPCs) to test their paracrine abilities. Currently I'm just use EBM-2 w/o growth factors and FBS to incubate my cells for...
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I induced apoptosis in my cell culture with 100uM CoCl2 (24 hours) but when I was trying to confirm the apoptosis by probing Caspase 8 in western blot I couldn't detect cleaved Caspase 8. Is there...
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Recently I read a paper talking about how cytoskeleton affects the function of bone marrow derived late EPCs. In this paper, late EPCs were seeded onto plates coated without any ECM or with...
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I'm working on myocardial infarction in mice and I'm going to stain the heart with Triphenyltetrazolium chloride (TTC). Could anyone kindly provide the detailed TTC staining protocol?
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Our lab purified some soluble protein and kept them at -80, but the concentration of the protein varies when we evaluate them again. Some of them increased while some of them decreased. I wonder...
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I isolated endothelial progenitor cells (EPCs) from mice bone marrow and cultured them in a human fibronectin coated plate for 4 weeks. The cells grew well in the plate albeit there were some...
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