Typically, clear cell renal cell carcinoma (CCRCC) is characterized by epithelial cells with clear cytoplasm and a well-defined cell membrane. Are there any software or methods we could use to quantify the transparency of the cytoplasm?
Have you tried to stain with alcianblue or mueller-mowry? This stains the proteoglycans in the clear part blue. Maybe an easier way to quantify the content.
Thank your for your reply. I have the images of HE stains. I am wondering if there were some software could analyze them and quantify the transparency of the cytoplasm.